Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Phengnoi, P.; Charoenwongpaiboon, T.; Wangpaiboon, K.; Klaewkla, M.; Nakapong, S.; Visessanguan, W.; Ito, K.; Pichyangkura, R.; Kuttiyawong, K.
    Levansucrase from Bacillus amyloliquefaciens KK9 and its Y237S variant producing the high bioactive levan-type fructooligosaccharides (2020), Biomolecules, 10, 692 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.10 expressed in Escherichia coli BL21 Star(DE3) cells Bacillus amyloliquefaciens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.10 Y237S the mutant efficiently produces a higher amount of short-chain levan-typed fructooligosaccharide than wild type with increased prebiotic activity Bacillus amyloliquefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.10 sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside Bacillus amyloliquefaciens
-
 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?
2.4.1.10 sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside Bacillus amyloliquefaciens KK9
-
 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Bacillus amyloliquefaciens M9TNM8
-
-
2.4.1.10 Bacillus amyloliquefaciens KK9 M9TNM8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10 CM Toyopearl column chromatography Bacillus amyloliquefaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.10 81.9
-
 crude enzyme, at pH 6.0 and 37°C Bacillus amyloliquefaciens
2.4.1.10 152.4
-
 after 1.9fold purification, at pH 6.0 and 37°C Bacillus amyloliquefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 sucrose + sucrose
-
 Bacillus amyloliquefaciens D-glucose + levan type-fructan
-
 ?
2.4.1.10 sucrose + sucrose
-
 Bacillus amyloliquefaciens KK9 D-glucose + levan type-fructan
-
 ?
2.4.1.10 sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
-
 Bacillus amyloliquefaciens D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?
2.4.1.10 sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
-
 Bacillus amyloliquefaciens KK9 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.10 ? x * 53000, SDS-PAGE Bacillus amyloliquefaciens
2.4.1.10 ? x * 5297, calculated from amino acid sequence Bacillus amyloliquefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 37
-
-
 Bacillus amyloliquefaciens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.10 20 50 more than 50% activity between 20 and 50°C Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.10 6
-
-
 Bacillus amyloliquefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.10 5 7 more than 60% activity between pH 5.0 and 7.0 Bacillus amyloliquefaciens